解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Real-time medical imaging systems such as reflectance confocal microscopes and optical coherence microscopes are being tested in multiple-patient and multiple-center clinical trials. The modulation transfer function (MTF) of these systems at any given time influences the image information content and can affect the in...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2779352
更新日期:2007-09-01 00:00:00
abstract::We investigate the feasibility of using diode laser gas spectroscopy for sinusitis diagnostics. We simulate light propagation using the Monte Carlo concept, as implemented by the Advanced Systems Analysis Program (ASAP) software. Simulations and experimental data are compared for a model based on two scattering bodies...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2777179
更新日期:2007-09-01 00:00:00
abstract::Studies of bioluminescence in living animals, such as cell-based biosensor applications, require measurement of light at different wavelengths, but accurate light measurement is impeded by absorption by tissues at wavelengths<600 nm. We present a novel approach to this problem--the use of a plastic window in the skin/...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2795567
更新日期:2007-09-01 00:00:00
abstract::Frequency domain optical coherence tomography (FD-OCT), based on an all-reflective high-speed InGaAs spectrometer, operating in the 1050 nm wavelength region for retinal diagnostics, enables high-speed, volumetric imaging of retinal pathologies with greater penetration into choroidal tissue is compared to conventional...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2773728
更新日期:2007-07-01 00:00:00
abstract::We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scanning modes, confocal r...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2753744
更新日期:2007-07-01 00:00:00
abstract::The study presents comparison of near-infrared light propagation and near-infrared backscattered radiation power, as simulated with numerical modeling and measured live in a patient in clinical conditions with the use of the near-infrared transillumination-backscattering sounding (NIR-TBSS) technique. A unique chance ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2753756
更新日期:2007-07-01 00:00:00
abstract::Raman spectroscopy on single, living epithelial cells captured in a laser trap is shown to have diagnostic power over colorectal cancer. This new single-cell technology comprises three major components: primary culture processing of human tissue samples to produce single-cell suspensions, Raman detection on singly tra...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2748060
更新日期:2007-05-01 00:00:00
abstract::We report a new two-channel fluorescence microscopy technique for surface-generated fluorescence. The realized fluorescence microscope allows high resolution imaging of aqueous samples. The core element of the instrument is a parabolic mirror objective that is used to collect the fluorescence at large surface angles a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2747627
更新日期:2007-05-01 00:00:00
abstract::We measure the tumor vascular response to varying irradiance rates during photodynamic therapy (PDT) in a Dunning rat prostate model with interstitial Doppler optical coherence tomography (IS-DOCT). Rats are given a photosensitizer drug, Photofrin, and the tumors are exposed to light (635 nm) with irradiance rates ran...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2744068
更新日期:2007-05-01 00:00:00
abstract::Angle-resolved signals of polarized light scattered by biological cells provide rich information on cell morphology. Quantitative study of these signals can lead to new methods to develop and improve high-throughput instrumentation for cell probing such as scattering-based flow cytometry. We employ a goniometer system...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2749730
更新日期:2007-05-01 00:00:00
abstract::We present methods for visualizing the dynamic response of biological samples to laser-induced heating. Our approach utilizes optical frequency-domain imaging to detect, spatially localize, and monitor unique dynamic signatures that arise within zones of active tissue denaturation. Since this information is precisely ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714027
更新日期:2007-03-01 00:00:00
abstract::The transmembrane protein prestin is crucial to outer hair cell (OHC) electromotility and contributes to the sensitivity and frequency selectivity of mammalian hearing. The molecular mechanisms of electromotility remain unclear, but prestin is purported to function as both a voltage sensor and a molecular motor. Under...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2715202
更新日期:2007-03-01 00:00:00
abstract::Mid-IR semiconductor lasers of two wavelength bands, 5.4 and 9.6 microm, are applied to measure aqueous glucose concentration ranging from 0 to 500 mgdL with Intralipid emulsion (0 to 8%) added as a fat simulator. The absorption coefficient micro(a) is found linear with respect to glucose and Intralipid concentrations...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714283
更新日期:2007-03-01 00:00:00
abstract::The acousto-optic deflector (AOD) is highly preferred in laser scanning microscopy for its fast scanning ability and random-addressing capability. However, its application in two-photon microscopy is frustrated by the dispersion of the AOD, which results in beam distortion and pulse lengthening. We report the analysis...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714061
更新日期:2007-03-01 00:00:00
abstract::Application of a fiber optic biosensor (FOB) to the real-time investigation of the interaction kinetics between FITC-conjugated monoclonal sheep anti-human C-reactive protein (CRP) antibody and CRP isoforms on the surface of optical fiber is described. Recently, both the native pentameric CRP (pCRP), an acute phase pr...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714029
更新日期:2007-03-01 00:00:00
abstract::Nonmelanoma skin cancer is the most common form of human cancer, often resulting in high morbidity. Low visual contrast of these tumors makes their delineation a challenging problem. Employing a linearly polarized monochromatic light source and a wide-field CCD camera, we have developed a technique for fluorescence po...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2435710
更新日期:2007-01-01 00:00:00
abstract::Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectionin...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2435626
更新日期:2007-01-01 00:00:00
abstract::Photodynamic therapy (PDT) is an alternative to radical surgical resection for T1a or nonresectable carcinomas of the gastroesophageal junction. Besides the concentration of the photosensitizer, the light distribution in tissue is responsible for tumor destruction. For this reason, knowledge about the behavior of ligh...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2564793
更新日期:2007-01-01 00:00:00
abstract::Optical imaging using voltage-sensitive dyes has become an important tool for studying vortex-like electrical waves in the heart. Such waves, known as spiral or scroll waves, can spontaneously form in pathological ventricular myocardium, causing ventricular fibrillation and sudden death. Until recently, observations o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2709661
更新日期:2007-01-01 00:00:00
abstract::Diffuse near-infrared tomography of tissue reveals scattering changes that originate from the submicroscopic features of the tissue; yet the existing tools to use this information to predict which features contribute to the scattering spectrum are limited by the lack of direct data quantifying the particle sizes. Brea...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2398903
更新日期:2006-11-01 00:00:00
abstract::As small animal optical imaging and tomography are gaining popularity for interrogating functional and molecular events in vivo, it becomes increasingly necessary to gain knowledge of the optical properties of the species investigated to better understand and describe photon propagation through their tissues. To achie...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2400702
更新日期:2006-11-01 00:00:00
abstract::We present Monte Carlo modeling studies to provide a quantitative understanding of contrast observed in spatially resolved reflectance spectra of normal and highly dysplastic cervical tissue. Simulations have been carried out to analyze the sensitivity of spectral measurements to a range of changes in epithelial and s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2398932
更新日期:2006-11-01 00:00:00
abstract::Pilot results on the application of the finite-difference time-domain (FDTD) approach for studying the implementation of the optical immersion technique for the visualization of single and multiple gold nanoparticles in biological cells are presented. We focus on two different scenarios considering single biological c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2400239
更新日期:2006-11-01 00:00:00
abstract::We employ Fourier-transform Raman spectroscopy to study normal and tumoral human breast tissues, including several subtypes of cancers. We analyzed 194 Raman spectra from breast tissues that were separated into 9 groups according to their corresponding histopathological diagnosis. The assignment of the relevant Raman ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2363362
更新日期:2006-09-01 00:00:00
abstract::We describe a computer eye model that allows for aspheric surfaces and a three-dimensional computer-based ray-tracing technique to simulate optical properties of the human eye and visual perception under various eye defects. Eye surfaces, such as the cornea, eye lens, and retina, are modeled or approximated by a set o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2357734
更新日期:2006-09-01 00:00:00
abstract::Type I collagen is a major component of the extracellular matrix as well as many tissue engineered models. To understand changes in collagen related models over time, it is important to evaluate collagen dynamics with noninvasive techniques. Fluorescence spectroscopy provides a method to noninvasively measure endogeno...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2360516
更新日期:2006-09-01 00:00:00
abstract::A need exists for the continued development of diagnostic tools and methods capable of distinguishing and characterizing slight differences in the optical properties of tissues. We present a method to estimate the scattering coefficient contribution as a function of particle size in complex mixtures of polystyrene sph...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2363347
更新日期:2006-09-01 00:00:00
abstract::Optical spectroscopy, imaging, and therapy tissue phantoms must have the scattering and absorption properties that are characteristic of human tissues, and over the past few decades, many useful models have been created. In this work, an overview of their composition and properties is outlined, by separating matrix, s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2335429
更新日期:2006-07-01 00:00:00
abstract::Selective retina treatment (SRT) is a novel short pulsed laser therapy of several retinal diseases associated with a decreased metabolism at the retinal pigment epithelium (RPE). The range of laser pulse energies is small, in which the desired selective RPE disintegration is achieved without adverse effects to the neu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2339815
更新日期:2006-07-01 00:00:00
abstract::Differential phase optical coherence tomography (DPOCT) is introduced to measure optical path length changes in response to pulsed laser irradiation (585 nm). An analytical equation that includes thermoelastic surface displacement and thermorefractive index change is derived to predict optical path length change in re...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2236289
更新日期:2006-07-01 00:00:00
abstract::We present a method for lung cancer detection exploiting reflectance spectra measured in vivo during endoscopic imaging of the lung. The measured reflectance spectra were analyzed using a specially developed light-transport model to obtain quantitative information about cancer-related, physiological, and morphologic c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2337529
更新日期:2006-07-01 00:00:00
abstract::In this study we examine the implications of excitation saturation on fluorescence recovery after photobleaching (FRAP) experiments. In particular we present both experimental and theoretical evidence that fluorescein, one of the most frequently used fluorophores in FRAP, does not always comply with the basic assumpti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2337531
更新日期:2006-07-01 00:00:00
abstract::Three major analytical tools in imaging science are summarized and demonstrated relative to optical imaging in vivo. Standard resolution testing is optimal when infinite contrast is used and hardware evaluation is the goal. However, deep tissue imaging of absorption or fluorescent contrast agents in vivo often present...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2209908
更新日期:2006-05-01 00:00:00
abstract::Under ultraviolet and visible light excitation, melanin is essentially a nonfluorescent substance. This work reports our study on near-infrared (NIR) fluorescence properties of melanins, and explores potential applications of NIR fluorescence techniques for evaluating skin disorders involving melanin. The NIR fluoresc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2204007
更新日期:2006-05-01 00:00:00
abstract::The absorption coefficient mu(a), scattering coefficient mu(s), and anisotropy factor g of diluted and undiluted human blood (hematocrit 0.84 and 42.1%) are determined under flow conditions in the wavelength range 250 to 1100 nm, covering the absorption bands of hemoglobin. These values are obtained by high precision ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2203659
更新日期:2006-05-01 00:00:00
abstract::Selective photothermal interaction using dye enhancement has proven to be effective in minimizing surrounding tissue damage and delivering energy to target tissue. During laser irradiation, the process of photon absorption and thermal energy diffusion in the target tissue and its surrounding tissue are crucial. Such i...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2204615
更新日期:2006-05-01 00:00:00
abstract::Heart disease is the primary cause of death in the western world. Many of these deaths are caused by the rupture of vulnerable plaque. Vulnerable plaques are characterized by a large lipid core covered by a thin fibrous cap. One method for detecting these plaques is reflection spectroscopy. Several studies have invest...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2186332
更新日期:2006-03-01 00:00:00
abstract::We fabricate scaffolds with a fine linear grating (periodicity of 1 to 8 microm and height of 1 microm) on a glass substrate for controlling cardiomyocyte orientation. The fabrication is done by the solidification of a liquid photopolymerizable material using two laser beam interference. As the photopolymerizable mate...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2186042
更新日期:2006-03-01 00:00:00
abstract::We report the design, integration, and validation of a fluorescence microscopy system for imaging of electroperturbation--the effects of nanosecond, megavolt-per-meter pulsed electric fields on biological cells and tissues. Such effects have potential applications in cancer therapy, gene regulation, and biophysical re...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2187970
更新日期:2006-03-01 00:00:00
abstract::A classifier capable of ranking structural alterations of the cytoskeleton is developed. Images of cytoskeletal microtubules obtained from the epifluorescence microscopy of primary culture rat hepatocytes are analyzed. Morphological descriptors are extracted by contour and mass fractal analysis, direct methods, and sp...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2187423
更新日期:2006-03-01 00:00:00